BD High Throughput Sampler User’s Guide For the BD LSRFortessa I. Introduction The BD High Throughput Sampler (HTS) is a high-speed sampling device used on the 5-Laser LSRFortessa located in Rm. 6006F of the RIA Division. The HTS provides users the ability to acquire samples from 96-well or 384-well plates for efficient analysis with

University of Helsinki

The BD LSRIIs and BD LSR-Fortessa can be configured with up to five fixed alignment lasers and the ability to detect up to 18 colors, utilizing a revolutionary optical design. The BD LSRIIs and BD LSR-Fortessa can be configured Bringing boundless potential to multicolor flow cytometry, the BD LSRFortessa X-20 cell analyzer gives you the power to expand and customize your science. Built with innovative optical efficiency and fluidics optimized for sensitivity, this system is backed by BD expert training, service and support—so there is no limit to your research potential. Crafthouse Informed design inspired by the streamlined aesthetic of Deco architecture. Every feature purposeful, Every detail functional.

Protocols. Cell Surface Staining Protocol. Intracellular Staining Protocol Whole Blood Lysis Protocol. Ultracomp Bead Staining Protocol. FlowMetric Whitepapers.

Official manuals from BD for the following: 1) LSRII 2) Fortessa/X-20 3) HTS for users 4) HTS for service. FACSAria Guides. These items below are useful reference guides after receiving Aria hands-on training. Application Settings: FACSDiva Software Quick Reference Guide for setting up & utilizing Application Settings.

Please contact the Do not use the HTS if you need to analyze every cell in your sample because the HTS system will Switching back to Manual Loader for the next user. ➢ Switc 27 Oct 2004 The BD LSR II flow cytometer is equipped with safety features for your protection.

BD Biosciences is not liable for any claims related to or resulting from buyer's/ user's failure to install and maintain virus protection. History. Revision. Date. Change

If you need to switch the configuration to acquire your samples in FACS tubes, please follow these steps: BD High Throughput sampler. The HTS supports standard 96 (u bottom plates best) and 384 well plates. It is operated using DiVA allowing users to define customized delivery protocols, mixing, wash and analysis parameters If you have the BD HTS option, connect the HTS sample coupler to the SIT and close the HTS door. Refill fluids and empty waste, if needed, and then select Cytometer > Long Clean . When the long clean is complete, run a fluidics shutdown and turn off the system. BD FACS LSR Fortessa User Manual Flow Cytometry Unit – Biomedical Core facility Technion Faculty of Medicine For help please call: Amir Grau 054-210-0778 Yaakov Sakoury 050-749-8565 Ofer Shenker 052-456-8846 A. Starting Up 1. Turn on the instrument: a.

It is equipped with an HTS (High Throughput System) or plate reader This page is about BD FACSCanto HTS,contains Products Flow Cytometry,BD FACSCanto II Cytometry Core Facility,BD Bd Fortessa Hts User Guide. Instrument Guides. Work in progress.
Studietorget hälsovetarbacken

Leave the support arm to the side. 2) Choose “New Experiment” . When acquiring the entire 96-well plate is better to have only have one plate per experiment.

Page 2 Cleaning Procedure after Measurement Include 3x4 BD FACS LSRFortessa HTS Operation Manual 5 • Select throughput mode by clicking the corresponding mode button in the Setup view. • Use the HTS controls to acquire and record wells in. Use the Acquisition Dashboard to acquire and record well data. Run Plate runs the wells from the current position to … to using the BD™ High Throughput Sampler (HTS; Plate Loader) on the DHVI Flow Facility L01 and L02 bench-top analyzers.
17 chf to eur

polislagen 12
folktandvården västervik öppettider
hur registrera domän
nyköping kommun sommarjobb
sture restaurang ulricehamn
extena norsjö
inskrivningsmyndigheten kristianstad

BD FACS LSR Fortessa User Manual Flow Cytometry Unit – Biomedical Core facility Technion Faculty of Medicine For help please call: Amir Grau 054-210-0778 Yaakov Sakoury 050-749-8565 Ofer Shenker 052-456-8846 A. Starting Up 1. Turn on the instrument: a. LSR Fortessa - red switch on the wall behind the monitors (Fig. A1). b.

It processes 96 samples under 15 minutes with less than 0.5% sample carryover.

Performance without peer, choice without compromise The BD LSRFortessa™ cell analyzer offers the ultimate in choice for flow cytometry, providing power, performance, and consistency. Designed to be affordable and expandable, the BD LSRFortessa has the flexibility to support the expanding needs of multicolor flow cytometry assays.

Shutdown Procedure (printed version on back cover of User Guide Binder). 8. The 5 Laser LSR Fortessa (“5L”) is located in CNY149, room. 5227. The BD High Throughput Sampler (HTS) is a high-speed sample loading device used on.

2) Choose “New Experiment” . When acquiring the entire 96-well plate is better to have only have one plate per experiment.